1990Journal of Dairy ScienceOpen access

Comparative Evaluation of Bulk Starter Substrates on Activity and Storage of Two Commercial Starter Strains

Tineke H. Jones, Lech Ozimek, Michael E. Stiles

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Abstract

Two out of five commercial strains of Lactococcus laclis ssp.cremoris were selected for their sensitivity or insensitivity to acidity based on growth and storage in 10% reconstituted skim milk.It was shown that both the acid-sensitive and acid-insensitive strains were protected when grown and stored in skim milk media with solids content increased by UF or by added solids (20% or 24% reconstituted skim milk).Internal pH medium was used as a reference for protective growth and storage of lactic starter cultures.Maintenance of activity over 5 d of storage at 2"C in 24% reconstituted skim milk and UF milk retentate (concentrated 3x) was better than or equal to internal pH medium for both strains of L. laclis ssp.cremoris.

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Two out of five commercial strains of Lactococcus laclis ssp.cremoris were selected for their sensitivity or insensitivity to acidity based on growth and storage in 10% reconstituted skim milk.It was shown that both the acid-sensitive and acid-insensitive strains were protected when grown and stored in skim milk media with solids content increased by UF or by added solids (20% or 24% reconstituted skim milk).Internal pH medium was used as a reference for protective growth and storage of lactic starter cultures.Maintenance of activity over 5 d of storage at 2"C in 24% reconstituted skim milk and UF milk retentate (concentrated 3x) was better than or equal to internal pH medium for both strains of L. laclis ssp.cremoris.

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Two out of five commercial strains of Lactococcus laclis ssp.cremoris were selected for their sensitivity or insensitivity to acidity based on growth and storage in 10% reconstituted skim milk.It was shown that both the acid-sensitive and acid-insensitive strains were protected when grown and stored in skim milk media with solids content increased by UF or by added solids (20% or 24% reconstituted skim milk).Internal pH medium was used as a reference for protective growth and storage of lactic starter cultures.Maintenance of activity over 5 d of storage at 2"C in 24% reconstituted skim milk and UF milk retentate (concentrated 3x) was better than or equal to internal pH medium for both strains of L. laclis ssp.cremoris.

Key concepts: Starter, Food science, Chemistry, Biology

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