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2011•Journal of Clinical MicrobiologyOpen access

Simultaneous Detection of Influenza A, Influenza B, and Respiratory Syncytial Viruses and Subtyping of Influenza A H3N2 Virus and H1N1 (2009) Virus by Multiplex Real-Time PCR

Yu Chen, Dawei Cui, Shufa Zheng, Shigui Yang, Jia Tong, Dagan Yang, Jian‐Gao Fan, Jie Zhang, Bin Lou, Xuefen Li, Xiaoling Zhuge, Bo Ye, Baode Chen, Weilin Mao, Yajun Tan, Gen-Yun Xu, Zhenjin Chen, Nan Chen, Lanjuan Li

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Abstract

A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

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A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

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Available abstract

A multiplex real-time PCR assay was developed to simultaneously detect and discriminate influenza A virus subtypes, including novel H1N1 (2009) and seasonal H3N2 virus, influenza B virus, and respiratory syncytial virus (RSV) in a single test tube, with detection sensitivity and specificity of 99% and 100%, respectively, for the four pathogens.

Key concepts: Subtyping, Virology, Virus, Multiplex, Influenza A virus, Multiplex polymerase chain reaction, Biology, Orthomyxoviridae

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Simultaneous Detection of Influenza A, Influenza B, and Respiratory Syncytial Viruses and Subtyping of Influenza A H3N2 Virus and H1N1 (2009) Virus by Multiplex Real-Time PCR — Research Paper | ScholarLens