Effects of kinetin and gibberellin a3 on callus growth and organ formation inLimnophila chinensis tissue culture

The action of exogenously applied hormones in the induction of morphogenesis in Limnophila chinensis (Osb.) Merr. tissue culture has been demonstrated. Stem expiants were grown on Murashige and Skoog’s medium containing various levels of kinetin, gibberellic acid and indole-3-acetic acid. Formation of roots, shoots (normal or abnormal), plantlets and friable, hard or nodulated calluses depended largely on the hormone levels used. The formation of normal shoots and roots were stimulated by treatment with kinetin. GA 3 treatment stimulated the bud differentiation but inhibited the root initiation. A combination of kinetin and GA 3 gave variable results.