1985•ElectrophoresisRequires access

Fabric reinforced polyacrylamide gels for electroblotting

Hideyuki Nishizawa, Ayako Murakami, Naoko Hayashi, Mami Iida, Yoshihiro Abe

Abstract

Abstract The preparation of 0.5 mm fabric reinforced polyacrylamide (FRP) gels is described. After isoelectric focusing, egg white and isoelectric point marker proteins were quantitatively transferred in only 20 min from the FRP gels to nitrocellulose membranes by electroblotting. FRP gels combine the advantages of previously described supports for polyacrylamide gels with penetrability for electric current during blotting. The gels may be conveniently stored and afford operational versatility.

About this research paper

What this paper is about

Why it matters

OpenAlex reports 16 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Electroblotting, Polyacrylamide, Nitrocellulose, Isoelectric focusing, Membrane, Isoelectric point, Polyacrylamide gel electrophoresis, Chromatography