1970•FEBS LettersRequires access

Purification of lactose synthetase a protein from human milk and demonstration of its interaction with α‐lactalbumin

P Andrews

Abstract

A fairly simple procedure for isolating lactose synthetase A protein from human milk in 36% yield is described. The A protein has a molecular weight of about 40,000. Its interaction with alpha-lactalbumin in the presence of N-acetylglucosamine has been demonstrated by affinity chromatography and gel filtration.

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Key concepts: Alpha-lactalbumin, Lactalbumin, Lactose, Size-exclusion chromatography, Chemistry, Whey protein, Chromatography, Yield (engineering)