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Human Aldehyde Dehydrogenase: Mechanism of Inhibition of Disulfiram

Robert C. Vallari, Regina Pietruszko

Abstract

Disulfiram labeled with carbon-14 reacts specifically with human liver aldehyde dehydrogenase E1 with loss of catalytic activity and no incorporation of label. Carbon-14-labeled diethyldithiocarbamate is formed and the number of enzyme sulfhydryl groups decreases from 34 to 30 during this process. Activity is recovered by-mercaptoethanol but not by glutathione, the physiological reducing agent.

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Key concepts: Disulfiram, Aldehyde dehydrogenase, Aldehyde, Chemistry, Enzyme, Glutathione, Biochemistry, Alcohol dehydrogenase