Resolution of natural hatching factors for golden potato cyst nematode, Globodera rostochiensis

Summary. The hatching responses of Globodera rostochiensis (golden potato cyst nematode) to purified and partially‐purified preparations of natural (including the potato glycoalkaloids solanine and α‐chaconine) and artificial hatching factors (HFs) were bimodal. At least 10 HFs, mostly anionic, were resolved from potato root leachate by a combination of gel permeation and ion‐exchange chromatography. Whereas potato roots were the principal source of HFs, haulm leachate also contained such chemicals. Root leachate from aseptically‐grown potato plants lacked several HFs which were present in conventionally‐produced leachate.