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Direct Assay of Tinidazole in Human Serum by Micellar Liquid Chromatography

D. Habel, S. Guermouche, M. H. Guermouche

Open publisher page 11 citations

Abstract

A liquid chromatographic procedure for the direct determination of tinidazole in human serum is presented. It includes the use of a micellar mobile phase consisting of SDS (5.10(-2) M): propan-1-ol; (94:6, v/v) and a mu Bondapak CN column with UV detection at 320 nm. No solvent extraction or deproteinization are necessary. The linearity (0.1-10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99%), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed-phase HPLC are used. One hundred injections of serum samples do not affect the column life. The procedure is applied to ascertain the pharmacokinetics of 10 mg/kg of tinidazole.

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What this paper is about

A liquid chromatographic procedure for the direct determination of tinidazole in human serum is presented. It includes the use of a micellar mobile phase consisting of SDS (5.10(-2) M): propan-1-ol; (94:6, v/v) and a mu Bondapak CN column with UV detection at 320 nm. No solvent extraction or deproteinization are necessary. The linearity (0.1-10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99%), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed-phase HPLC are used. One hundred injections of serum samples do not affect the column life. The procedure is applied to ascertain the pharmacokinetics of 10 mg/kg of tinidazole.

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Available abstract

A liquid chromatographic procedure for the direct determination of tinidazole in human serum is presented. It includes the use of a micellar mobile phase consisting of SDS (5.10(-2) M): propan-1-ol; (94:6, v/v) and a mu Bondapak CN column with UV detection at 320 nm. No solvent extraction or deproteinization are necessary. The linearity (0.1-10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99%), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed-phase HPLC are used. One hundred injections of serum samples do not affect the column life. The procedure is applied to ascertain the pharmacokinetics of 10 mg/kg of tinidazole.

Key concepts: Tinidazole, Chromatography, Chemistry, Micellar liquid chromatography, High-performance liquid chromatography, Detection limit, Extraction (chemistry), Reproducibility

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