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A SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF FUROCOUMARIN CONTENT OF THE FRUITS OFAMMI MAJUS

S. Balbaa, Samsu Hilal, M. Haggag

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Abstract

A TLC–spectrophotometric method was proposed for the estimation of xanthotoxin, imperatorin and bergapten, i. e., the main furocoumarins present in the fruits of Ammi majus. This method depends on separating these furocoumarins by TLC, followed by their elution, and spectrophotometric determination. Calculations were made referring to previously prepared standard curves. The sensitivity of the method was confirmed by determining the percentage recoveries of xanthotoxin, imperatorin and bergapten and which were found to be 99.7–102.2, 96.17–98.1 and 97.3–98.2 respectively.

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What this paper is about

A TLC–spectrophotometric method was proposed for the estimation of xanthotoxin, imperatorin and bergapten, i. e., the main furocoumarins present in the fruits of Ammi majus. This method depends on separating these furocoumarins by TLC, followed by their elution, and spectrophotometric determination. Calculations were made referring to previously prepared standard curves. The sensitivity of the method was confirmed by determining the percentage recoveries of xanthotoxin, imperatorin and bergapten and which were found to be 99.7–102.2, 96.17–98.1 and 97.3–98.2 respectively.

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Available abstract

A TLC–spectrophotometric method was proposed for the estimation of xanthotoxin, imperatorin and bergapten, i. e., the main furocoumarins present in the fruits of Ammi majus. This method depends on separating these furocoumarins by TLC, followed by their elution, and spectrophotometric determination. Calculations were made referring to previously prepared standard curves. The sensitivity of the method was confirmed by determining the percentage recoveries of xanthotoxin, imperatorin and bergapten and which were found to be 99.7–102.2, 96.17–98.1 and 97.3–98.2 respectively.

Key concepts: Furocoumarins, Bergapten, Imperatorin, Furocoumarin, Chromatography, Psoralen, Chemistry, Elution

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A SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF FUROCOUMARIN CONTENT OF THE FRUITS OFAMMI MAJUS — Research Paper | ScholarLens