A high-throughput assay for the adenylation reaction of bacterial DNA ligase
Lynn Miesel, Cynthia Kravec, Alan-Tianpei Xin, Patricia McMonagle, Sarah Ma, John Pichardo, Boris Feld, Ellen B. Barrabee, Robert E. Palermo
Abstract
Lynn Miesel, Cynthia Kravec, Alan-Tianpei Xin, Patricia McMonagle, Sarah Ma, John Pichardo, Boris Feld, Ellen B. Barrabee, Robert E. Palermo
Abstract
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Key concepts: DNA ligase, Okazaki fragments, Phosphodiester bond, DNA Ligases, Adenylylation, Biochemistry, NAD+ kinase, Biology