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Transfection with the Proviral DNA of a Type D Retrovirus (Mason-Pfizer Monkey Virus)

Hajime Ogura, Tazuko Fujiwara, Hideo Takechi

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Abstract

Chromosomal DNA was isolated from CMMT cells which were producing Mason-Pfizer monkey virus (M-PMV), a type D simian retrovirus. Human embryonic cells were transfected with the DNA. About 2 weeks after transfection, 3 of 7 culture dishes began to produce M-PMV which could be detected by virus-mediated cell fusion in RSb human cells dually transformed by Rous sarcoma virus and simian virus 40, reverse transcriptase assay, and electron microscopy. Mock-transfected cultures did not produce virus.

About this research paper

What this paper is about

Chromosomal DNA was isolated from CMMT cells which were producing Mason-Pfizer monkey virus (M-PMV), a type D simian retrovirus. Human embryonic cells were transfected with the DNA. About 2 weeks after transfection, 3 of 7 culture dishes began to produce M-PMV which could be detected by virus-mediated cell fusion in RSb human cells dually transformed by Rous sarcoma virus and simian virus 40, reverse transcriptase assay, and electron microscopy. Mock-transfected cultures did not produce virus.

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Available abstract

Chromosomal DNA was isolated from CMMT cells which were producing Mason-Pfizer monkey virus (M-PMV), a type D simian retrovirus. Human embryonic cells were transfected with the DNA. About 2 weeks after transfection, 3 of 7 culture dishes began to produce M-PMV which could be detected by virus-mediated cell fusion in RSb human cells dually transformed by Rous sarcoma virus and simian virus 40, reverse transcriptase assay, and electron microscopy. Mock-transfected cultures did not produce virus.

Key concepts: Virology, Rous sarcoma virus, Retrovirus, Transfection, Virus, Biology, Simian, Provirus

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Transfection with the Proviral DNA of a Type D Retrovirus (Mason-Pfizer Monkey Virus) — Research Paper | ScholarLens