2010•Artificial Cells Blood Substitutes and BiotechnologyOpen access

Biochemical Properties of Free and ImmobilizedCandida rugosaLipase onto Al2O3: A Comparative Study

Yeşim Yeşiloğlu, Latifşah Şit

Abstract

Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.

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Key concepts: Candida rugosa, Lipase, Immobilized enzyme, Adsorption, Enzyme, Chemistry, Yield (engineering), Chromatography