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1985•Journal of Liquid ChromatographyRequires access

High Pressure Liquid Chromato-Graphic Separation of Aryl-Naphthalide Lignan Lactones

K. S. Annapoorani, C. Damodaran, P. Chandra Sekharan

Open publisher page 6 citations

Abstract

A high-pressure liquid chromatographic system is described for the separation of few arylnaphthalide lignan lactones. An octadecyl-silica (Spherisorb 5 ODS) column was used with methanol/water (73/27 volume %) isocratic system as eluent. Both HPLC-fluorometry and HPLC-UV detection methods were employed. The present technique is compared with other instrumental methods developed earlier and its utility in chemotaxonomic and toxicologic studies is discussed.

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What this paper is about

A high-pressure liquid chromatographic system is described for the separation of few arylnaphthalide lignan lactones. An octadecyl-silica (Spherisorb 5 ODS) column was used with methanol/water (73/27 volume %) isocratic system as eluent. Both HPLC-fluorometry and HPLC-UV detection methods were employed. The present technique is compared with other instrumental methods developed earlier and its utility in chemotaxonomic and toxicologic studies is discussed.

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Available abstract

A high-pressure liquid chromatographic system is described for the separation of few arylnaphthalide lignan lactones. An octadecyl-silica (Spherisorb 5 ODS) column was used with methanol/water (73/27 volume %) isocratic system as eluent. Both HPLC-fluorometry and HPLC-UV detection methods were employed. The present technique is compared with other instrumental methods developed earlier and its utility in chemotaxonomic and toxicologic studies is discussed.

Key concepts: Chromatography, Lignan, High-performance liquid chromatography, Chemistry, Methanol, Column chromatography, Fluorescence spectroscopy, Silica gel

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