ScholarLens Back to search
1977•Immunological CommunicationsRequires access

The Influence of Thymosin on Affinity Binding in the E-Rosette Assay

Donald B. Kaufman, Carlos R. Sledge, M. Knust

Open publisher page 6 citations

Abstract

Human peripheral blood lymphocytes (PBL) were analyzed in the E rosette assay with respect to (1) the effect of SRBC/lymphocyte ratio on rosette number; (2) the effect of this ratio on the presence of lymphocytes with multiple binding sites for SRBC; and (3) the influence of thymosin on increasing the number of lymphocyte receptors for SRBC. An increase in the SRBC/lymphocyte ratio was associated with an increase in rosette number. The number of multiple binding rosettes was also dependent on the SRBC/lymphocyte ratio. At saturing SRBC concentrations, greater than 30% of the rosettes were in the morula form. Treatment of PBL with thymosin was associated with a slight increase in the number of total rosettes and a large increase in number of morulas. It is suggested that the morula represents a mature form of T cell in contrast to the 2 - 3 SRBC rosette, which may be induced by thymosin to become a morula form.

About this research paper

What this paper is about

Human peripheral blood lymphocytes (PBL) were analyzed in the E rosette assay with respect to (1) the effect of SRBC/lymphocyte ratio on rosette number; (2) the effect of this ratio on the presence of lymphocytes with multiple binding sites for SRBC; and (3) the influence of thymosin on increasing the number of lymphocyte receptors for SRBC. An increase in the SRBC/lymphocyte ratio was associated with an increase in rosette number. The number of multiple binding rosettes was also dependent on the SRBC/lymphocyte ratio. At saturing SRBC concentrations, greater than 30% of the rosettes were in the morula form. Treatment of PBL with thymosin was associated with a slight increase in the number of total rosettes and a large increase in number of morulas. It is suggested that the morula represents a mature form of T cell in contrast to the 2 - 3 SRBC rosette, which may be induced by thymosin to become a morula form.

Why it matters

OpenAlex reports 6 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Human peripheral blood lymphocytes (PBL) were analyzed in the E rosette assay with respect to (1) the effect of SRBC/lymphocyte ratio on rosette number; (2) the effect of this ratio on the presence of lymphocytes with multiple binding sites for SRBC; and (3) the influence of thymosin on increasing the number of lymphocyte receptors for SRBC. An increase in the SRBC/lymphocyte ratio was associated with an increase in rosette number. The number of multiple binding rosettes was also dependent on the SRBC/lymphocyte ratio. At saturing SRBC concentrations, greater than 30% of the rosettes were in the morula form. Treatment of PBL with thymosin was associated with a slight increase in the number of total rosettes and a large increase in number of morulas. It is suggested that the morula represents a mature form of T cell in contrast to the 2 - 3 SRBC rosette, which may be induced by thymosin to become a morula form.

Key concepts: Thymosin, Rosette (schizont appearance), Rosette formation, Lymphocyte, Receptor, Molecular biology, Biology, T lymphocyte

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
The Influence of Thymosin on Affinity Binding in the E-Rosette Assay — Research Paper | ScholarLens