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1998•FEMS Microbiology EcologyRequires access

Characterization of rhizobia homologues of Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4

Pablo J. Villadas, Pedro Burgos, Dulce N Rodrı́guez-Navarro, Francisco Temprano, Nicolás Toro

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Abstract

Homologues to Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4 were found within Rhizobium leguminosarum bv. viciae field populations. Similarly, homologous sequences to S. meliloti ISRm4 were found in S. fredii strains. Based on the polymerase chain reaction, we cloned and partially sequenced some of these putative insertion sequence elements. DNA sequence comparisons indicate that S. meliloti and R. leguminosarum ISRm3-type elements are closely related whereas the ISRm4 homologues show distinct relationships. In addition, specific primers for PCR recognition of ISRm3 and ISRm4 elements in rhizobia species and S. meliloti ISRm6 were designed.

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What this paper is about

Homologues to Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4 were found within Rhizobium leguminosarum bv. viciae field populations. Similarly, homologous sequences to S. meliloti ISRm4 were found in S. fredii strains. Based on the polymerase chain reaction, we cloned and partially sequenced some of these putative insertion sequence elements. DNA sequence comparisons indicate that S. meliloti and R. leguminosarum ISRm3-type elements are closely related whereas the ISRm4 homologues show distinct relationships. In addition, specific primers for PCR recognition of ISRm3 and ISRm4 elements in rhizobia species and S. meliloti ISRm6 were designed.

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Available abstract

Homologues to Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4 were found within Rhizobium leguminosarum bv. viciae field populations. Similarly, homologous sequences to S. meliloti ISRm4 were found in S. fredii strains. Based on the polymerase chain reaction, we cloned and partially sequenced some of these putative insertion sequence elements. DNA sequence comparisons indicate that S. meliloti and R. leguminosarum ISRm3-type elements are closely related whereas the ISRm4 homologues show distinct relationships. In addition, specific primers for PCR recognition of ISRm3 and ISRm4 elements in rhizobia species and S. meliloti ISRm6 were designed.

Key concepts: Sinorhizobium meliloti, Biology, Rhizobium leguminosarum, Rhizobia, Rhizobiaceae, Rhizobium, Insertion sequence, Genetics

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Characterization of rhizobia homologues of Sinorhizobium meliloti insertion sequences ISRm3 and ISRm4 — Research Paper | ScholarLens