1974•Canadian Journal of BiochemistryRequires access

Catalysis of Exchange of Oxygen Into and Out of L -Leucine by Leucine Aminopeptidase

James A. Wells, Gary L. Glasser, Frederick H. Carpenter

Abstract

Leucine aminopeptidase (L-leucyl-peptide hydrolase EC 3.4.1.1) was shown to catalyze the exchange of oxygen-18 into and out of L-leucine. Incubation of L-leucine at 30° with Mg2+-activated leucine aminopeptidase in a solution that was enriched in oxygen-18 containing water for 24 h at pH 7.0 resulted in a substantial increase in the oxygen-18 content of the L-leucine as compared with blanks. When L-leucine, enriched with oxygen-18, was treated similarly with leucine aminopeptidase, a significant decrease in the oxygen-18 content occurred as compared with blank solutions and a heat-denatured leucine aminopeptidase control.

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Key concepts: Aminopeptidase, Leucine, Chemistry, Oxygen, Biochemistry, Leucyl aminopeptidase, Peptide, Amino acid