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Effect of Ethylenediaminetetraacetic Acid (EDTA) and Metal Ions on Growth of Staphylococcus aureus 196E in Culture Media

Janice M. Kraniak, Leora A. Shelef

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Abstract

ABSTRACT The effects of ethylenediaminetetraacetic acid (EDTA) and its salts, alone or in combination with Mg 2+ , Fe 3+ , Zn 2+ or Ca 2+ , on growth of S. aureus strain 196E, were studied. EDTA or its Na + or K + salts (0.8–1.7 mM), but not its Ca 2+ or Fe 3+ salts, inhibited cell growth in Brain Heart Infusion broth. Bacteriostatic effects in Casamino Acids‐Yeast Extract (CasY) broth were produced by 0.17 mM EDTA. Addition of Fe 3+ , Zn 2+ , or Ca 2+ to EDTA‐containing broth eliminated inhibition at an EDTA: cation molar ratio of 1:1, while Mg 2+ required a sevenfold concentration. Data suggest that EDTA exerts its inhibitory effect by chelating calcium and/or other essential cations which form complexes with comparable stability constants with EDTA.

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ABSTRACT The effects of ethylenediaminetetraacetic acid (EDTA) and its salts, alone or in combination with Mg 2+ , Fe 3+ , Zn 2+ or Ca 2+ , on growth of S. aureus strain 196E, were studied. EDTA or its Na + or K + salts (0.8–1.7 mM), but not its Ca 2+ or Fe 3+ salts, inhibited cell growth in Brain Heart Infusion broth. Bacteriostatic effects in Casamino Acids‐Yeast Extract (CasY) broth were produced by 0.17 mM EDTA. Addition of Fe 3+ , Zn 2+ , or Ca 2+ to EDTA‐containing broth eliminated inhibition at an EDTA: cation molar ratio of 1:1, while Mg 2+ required a sevenfold concentration. Data suggest that EDTA exerts its inhibitory effect by chelating calcium and/or other essential cations which form complexes with comparable stability constants with EDTA.

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Available abstract

ABSTRACT The effects of ethylenediaminetetraacetic acid (EDTA) and its salts, alone or in combination with Mg 2+ , Fe 3+ , Zn 2+ or Ca 2+ , on growth of S. aureus strain 196E, were studied. EDTA or its Na + or K + salts (0.8–1.7 mM), but not its Ca 2+ or Fe 3+ salts, inhibited cell growth in Brain Heart Infusion broth. Bacteriostatic effects in Casamino Acids‐Yeast Extract (CasY) broth were produced by 0.17 mM EDTA. Addition of Fe 3+ , Zn 2+ , or Ca 2+ to EDTA‐containing broth eliminated inhibition at an EDTA: cation molar ratio of 1:1, while Mg 2+ required a sevenfold concentration. Data suggest that EDTA exerts its inhibitory effect by chelating calcium and/or other essential cations which form complexes with comparable stability constants with EDTA.

Key concepts: Ethylenediaminetetraacetic acid, Chemistry, Chelation, Nuclear chemistry, Metal, Metal ions in aqueous solution, Calcium, Staphylococcus aureus

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Effect of Ethylenediaminetetraacetic Acid (EDTA) and Metal Ions on Growth of Staphylococcus aureus 196E in Culture Media — Research Paper | ScholarLens