2013•Korean Journal of Medicinal Crop ScienceRequires access

Effect of Sucrose and Boric Acid on In Vitro Pollen Germination of Boxthorn (Lycium chinense M.)

Su Dong Kim, Byung‐Hoon Min, Wha Mo Yoon, Ok Ran Lee

Abstract

This study was carried out to investigate the effect of sucrose, boric acid, and calcium nitrate in optimum pH and relative humidity on pollen germination and pollen tube growth of boxthorn (Lycium chinense M.). Medium containing 100㎎/L of H3BO3 showed maximum pollen germination and pollen tube elongation, 64.8% and 920㎛. Additionally supplied calcium nitrate to the medium lowered pollen germination rate and pollen tube elongation. Here we report the optimum conditions for the pollen germination and pollen tube development was observed at 10% sucrose, 1.0% agar, pH 6.0, and 100% relative humidity.

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Key concepts: Germination, Pollen, Pollen tube, Calcium nitrate, Boric acid, Sucrose, Relative humidity, Elongation