Volatile antifungal substances emitted from bacterial culture.
Tadayoshi Tanaka, Zenya Shoji
Abstract
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Tadayoshi Tanaka, Zenya Shoji
Abstract
Open-access reader
The volatile antifungal substances produced by bacteria were researched by the double Petri-dish assay. The double Petri-dish was composed of an inner small dish inoculated with a microbial strain as a test organism and a larger Petri-dish containing another microbial strain culture previously grown on agar medium as a producing organism. In this study, 17 bacterial strains, 2 actinomycetes strains, 3 yeast strains and 5 fungal strains were used as volatile substance-producing organisms and its test organisms. In most cases, the growth of fungi as test organism was delayed or inhibited completely when a bacterium had been cultured in the large dish as the producer except lactic acid bacteria. In the assay system, producer and test strains are communicated through the gas phase only. The delay or inhibition of the growth of fungi can be presumed as a result caused by volatile substances produced by the producer bacteria
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The volatile antifungal substances produced by bacteria were researched by the double Petri-dish assay. The double Petri-dish was composed of an inner small dish inoculated with a microbial strain as a test organism and a larger Petri-dish containing another microbial strain culture previously grown on agar medium as a producing organism. In this study, 17 bacterial strains, 2 actinomycetes strains, 3 yeast strains and 5 fungal strains were used as volatile substance-producing organisms and its test organisms. In most cases, the growth of fungi as test organism was delayed or inhibited completely when a bacterium had been cultured in the large dish as the producer except lactic acid bacteria. In the assay system, producer and test strains are communicated through the gas phase only. The delay or inhibition of the growth of fungi can be presumed as a result caused by volatile substances produced by the producer bacteria
Key concepts: Petri dish, Bacteria, Microbiology, Organism, Agar, Biology, Agar plate, Yeast