Comparison of Two Chemicals for Removing an Entocytherid (Ostracoda: Crustacea) Species from Its Host Crayfish (Cambaridae: Crustacea)

Abstract Entocytherids are epifauna on larger crustaceans. We assessed the effectiveness of chlorobutanol and carbonated water for removing entocytherid, Ankylocythere sinuosa, from crayfish, Procambarus clarkii. This was done using different exposure times and by assessing entocytherid and crayfish survival, together with the number of entocytherids removed. According to our results, chlorobutanol submersion of crayfishes in shorter time periods is more effective for removing entocytherids without killing the crayfish host. However, carbonated water submersion is better to extract larger amounts of living entocytherids. Finally, both chemicals are appropriate tools to establish standardised protocols for removing entocytherids from crayfish for ecological studies because the proportions of removed entocytherids per crayfish become constant whatever the entocytherid load of the crayfish. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)