ScholarLens Back to search
1995•FEBS LettersOpen access

Extension of DNA triple helix formation to a neighbouring (AT)n site

Simon P. Chandler, Keith R. Fox

Open full text 12 citations

Abstract

We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A11(AT)6.(AT)6T11, using oligonucleotides designed to form parallel T.AT and G.TA triplets. We find that, although (TG)6 does not form a complex with (AT)6.(AT)6, T11(TG)6 forms a stable structure producing a clear footprint which includes the (AT)6 portion of the target site. This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a triplex-binding ligand.

Open-access reader

About this research paper

What this paper is about

We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A11(AT)6.(AT)6T11, using oligonucleotides designed to form parallel T.AT and G.TA triplets. We find that, although (TG)6 does not form a complex with (AT)6.(AT)6, T11(TG)6 forms a stable structure producing a clear footprint which includes the (AT)6 portion of the target site. This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a triplex-binding ligand.

Why it matters

OpenAlex reports 12 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

We have used DNase I footprinting to examine the formation of intermolecular triple helices at a fragment containing the target sequence A11(AT)6.(AT)6T11, using oligonucleotides designed to form parallel T.AT and G.TA triplets. We find that, although (TG)6 does not form a complex with (AT)6.(AT)6, T11(TG)6 forms a stable structure producing a clear footprint which includes the (AT)6 portion of the target site. This complex is not formed in the presence of magnesium, but can be stabilised by either manganese or a triplex-binding ligand.

Key concepts: Triple helix, Footprinting, Oligonucleotide, Chemistry, Manganese, Ligand (biochemistry), Helix (gastropod), Stereochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Extension of DNA triple helix formation to a neighbouring (AT)n site — Research Paper | ScholarLens