1984•Journal of Testing and EvaluationRequires access

Evaluation of the Ultrasonic Method for Solubilizing Dapnia magna Before Liquid Scintillation Counting

D.D. Dauble, RW Hanf, DW Carlile

Abstract

Abstract Adult Daphnia magna were exposed to 14C-labeled phenol and tissues analyzed for 14C uptake by three methods: (1) tissue solubilizer, (2) tissue solubilizer plus sonication, and (3) sonication alone. Analysis by liquid scintillation counting revealed that measurements of total activity among treatments were not significantly different (α ≤ 0.10) at two count levels. Sonicated samples showed less variation than tissue samples that were solubilized.

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Available abstract

Key concepts: Sonication, Liquid scintillation counting, Solubilization, Daphnia magna, Chromatography, Chemistry, Phenol, Ultrasonic sensor