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Simultaneous determination of atropine and scopolamine in plants by mixed‐column high performance liquid chromatography

Soumyajit Mandal, A. A. Naqvi, Raghunath S. Thakur

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Abstract

Abstract This paper describes a simple and rapid high performance liquid chromatographic method using two different polarity columns in series for the quantitative analysis of the major tropane alkaloids atropine and scopolamine. The problems associated with the simultaneous determination of the two alkaloids in plant extracts have been overcome. The method has been successfully applied to the analysis of three species of Solanaceous plants.

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What this paper is about

Abstract This paper describes a simple and rapid high performance liquid chromatographic method using two different polarity columns in series for the quantitative analysis of the major tropane alkaloids atropine and scopolamine. The problems associated with the simultaneous determination of the two alkaloids in plant extracts have been overcome. The method has been successfully applied to the analysis of three species of Solanaceous plants.

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Available abstract

Abstract This paper describes a simple and rapid high performance liquid chromatographic method using two different polarity columns in series for the quantitative analysis of the major tropane alkaloids atropine and scopolamine. The problems associated with the simultaneous determination of the two alkaloids in plant extracts have been overcome. The method has been successfully applied to the analysis of three species of Solanaceous plants.

Key concepts: Chemistry, Tropane, Scopolamine, Chromatography, Atropine, High-performance liquid chromatography, Polarity (international relations), Organic chemistry

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