2003•Biotechnology and Applied BiochemistryRequires access

Purification and properties of a transglutaminase produced by a Bacillus circulans strain isolated from the Amazon environment

Luís Henrique de Barros Soares, Francine Assmann, Marco Antônio Záchia Ayub

Abstract

A new microbial transglutaminase (EC 2.3.2.13) from a Bacillus circulans strain isolated from the aquatic Amazonian environment was purified and characterized. Enzyme purification started with (NH(4))(2)SO(4) 'salting out' and proceeded with liquid chromatography on Q-Sepharose FF and octyl-Sepharose 4 FF. The purification factor was approx. 150-fold with a yield of 32%. The enzyme's molecular mass was estimated as 45000 Da on SDS/PAGE. The purified transglutaminase had an optimum temperature of 47 degrees C, the optimum pH of the reaction was 7 and it presented no calcium-dependent activity.

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Key concepts: Bacillus circulans, Tissue transglutaminase, Salting out, Bacillales, Bacillaceae, Enzyme, Sepharose, Strain (injury)