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2008•Journal of Analytical ChemistryRequires access

Detection of DNA depurination with the use of an electrode modified with carbon nanotubes

Timur I. Abdullin, I. I. Nikitina, Oksana V. Bondar

Open publisher page 5 citations

Abstract

The possibility of detecting DNA depurination products on a glassy carbon electrode modified with carbon nanotubes (CNTs) was revealed. Adenine and a guanine DNA nucleotide were oxidized at similar potentials of about +1.1 V against Ag/AgCl; this fact did not allow us to detect adenine in the presence of DNA. DNA components did not interfere with the detection of guanine, which was oxidized at +0.9 V. Guanine was strongly adsorbed on the CNT-modified glassy carbon electrode; the oxidation current of guanine was a linear function of the concentration described by the equation i (10 −6 A) = 3.21 + 0.44 c (10 −6 M), r = 0.963. The results were suitable for the determination of guanine liberated from DNA on the CNT-modified glassy carbon electrode. The procedure developed was applied to evaluate the rates of DNA depurination under various conditions.

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What this paper is about

The possibility of detecting DNA depurination products on a glassy carbon electrode modified with carbon nanotubes (CNTs) was revealed. Adenine and a guanine DNA nucleotide were oxidized at similar potentials of about +1.1 V against Ag/AgCl; this fact did not allow us to detect adenine in the presence of DNA. DNA components did not interfere with the detection of guanine, which was oxidized at +0.9 V. Guanine was strongly adsorbed on the CNT-modified glassy carbon electrode; the oxidation current of guanine was a linear function of the concentration described by the equation i (10 −6 A) = 3.21 + 0.44 c (10 −6 M), r = 0.963. The results were suitable for the determination of guanine liberated from DNA on the CNT-modified glassy carbon electrode. The procedure developed was applied to evaluate the rates of DNA depurination under various conditions.

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Available abstract

The possibility of detecting DNA depurination products on a glassy carbon electrode modified with carbon nanotubes (CNTs) was revealed. Adenine and a guanine DNA nucleotide were oxidized at similar potentials of about +1.1 V against Ag/AgCl; this fact did not allow us to detect adenine in the presence of DNA. DNA components did not interfere with the detection of guanine, which was oxidized at +0.9 V. Guanine was strongly adsorbed on the CNT-modified glassy carbon electrode; the oxidation current of guanine was a linear function of the concentration described by the equation i (10 −6 A) = 3.21 + 0.44 c (10 −6 M), r = 0.963. The results were suitable for the determination of guanine liberated from DNA on the CNT-modified glassy carbon electrode. The procedure developed was applied to evaluate the rates of DNA depurination under various conditions.

Key concepts: Depurination, Guanine, Chemistry, DNA, Carbon nanotube, Electrode, Glassy carbon, Nucleobase

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