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1991•Proceedings of the Japan Academy Series BOpen access

Factors Required for Differentiation of Chick Precardiac Mesoderm Cultured in vitro.

Yoshihito Yamazaki, Reiji Hirakow

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Abstract

Precardiac mesoderm (PCM) of chick embryo at stage 5 was excised with or without endoderm and ectoderm and cultured in vitro using Eagle's minimum essential medium (MEM) as a basic medium. PCM placed alone in MEM degenerated. When PCM was associated with endoderm, it produced beating tissue in MEM, while it did not show pulsation with ectoderm in the same condition. If MEM was supplemented with 15% horse serum, PCM developed into beating tissue without endoderm. Insulin (25μg/ml) was effective in differentiation of PCM to myocardial cells.

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Precardiac mesoderm (PCM) of chick embryo at stage 5 was excised with or without endoderm and ectoderm and cultured in vitro using Eagle's minimum essential medium (MEM) as a basic medium. PCM placed alone in MEM degenerated. When PCM was associated with endoderm, it produced beating tissue in MEM, while it did not show pulsation with ectoderm in the same condition. If MEM was supplemented with 15% horse serum, PCM developed into beating tissue without endoderm. Insulin (25μg/ml) was effective in differentiation of PCM to myocardial cells.

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Available abstract

Precardiac mesoderm (PCM) of chick embryo at stage 5 was excised with or without endoderm and ectoderm and cultured in vitro using Eagle's minimum essential medium (MEM) as a basic medium. PCM placed alone in MEM degenerated. When PCM was associated with endoderm, it produced beating tissue in MEM, while it did not show pulsation with ectoderm in the same condition. If MEM was supplemented with 15% horse serum, PCM developed into beating tissue without endoderm. Insulin (25μg/ml) was effective in differentiation of PCM to myocardial cells.

Key concepts: Endoderm, Mesoderm, Ectoderm, FGF and mesoderm formation, NODAL, Embryo, In vitro, Germ layer

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