Distribution of Different Alleles of Aromatase Cytochrome P450 (CYP19) and Melatonin Receptor 1A (MTRN1A)Genes among Native Turkish Sheep Breeds

In this study, 257 animals from eight native Turkish sheep breeds (Akkaraman, Daglic, Gokceada, Ivesi, Karacabey Merino, Karayaka, Kivircik, Sakiz) were investigated for three SNPs located at CYP19 and MTRN1A genes which are important for reproductive traits. The A -> G transition within CYP19, the C -> T and G -> A transitions within MTRN1A were investigated by using PCR-RFLP method. To analyze CYP19 locus DraI, for MTRN1A locus MnlI and RsaI restriction enzymes were used. Two alleles were found for all the loci investigated. These alleles named as A and G for CYP19, M and m for MTRN1A-MnlI, and R and r for MTRN1A-Rsal. A and m alleles were predominant at CYP19 and MTRN1A-MnlI loci, respectively. While R allel was prevalent in majority of breeds investigated, r allele was predominant in Sakiz, Karacabey Merino, and Karayaka breeds for MTRN1A-Rsal. UPGMA test results show that the allele frequencies distributions of these loci can be used for calculating genetic distance between breeds.