Auxin content and growth patterns in auxin-dependent and auxin-autotrophic plant cell and tissue cultures
David M. Mousdale, Catherine Fidgeon, Graham Wilson
Abstract
David M. Mousdale, Catherine Fidgeon, Graham Wilson
Abstract
Endogenous concentrations of indol-3-ylacetic acid (IAA) in auxin-autotrophio crown gall tissue cultures are presented in relation to culture growth rate and to tissue IAA levels in auxin-dependent cultures maintained on IAA as auxin source. Cellular levels of synthetic auxins absorbed from culture media were monitored in auxin-dependent cell cultures exhibiting selectivity in growth response to exogenous auxins. The results are used in modelling culture growth patterns and changes in intracellular auxin content.
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Endogenous concentrations of indol-3-ylacetic acid (IAA) in auxin-autotrophio crown gall tissue cultures are presented in relation to culture growth rate and to tissue IAA levels in auxin-dependent cultures maintained on IAA as auxin source. Cellular levels of synthetic auxins absorbed from culture media were monitored in auxin-dependent cell cultures exhibiting selectivity in growth response to exogenous auxins. The results are used in modelling culture growth patterns and changes in intracellular auxin content.
Key concepts: Auxin, Biology, Tissue culture, Endogeny, Cell biology, Cell culture, Botany, Cell growth