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1978•Cold Spring Harbor Symposia on Quantitative BiologyRequires access

Characterization of DNase-I Cleavage Sites in the Nucleosome

Leonard C. Lutter

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Abstract

The use of nucleases as structural probes has proven to be a very successful approach in studying the organization of chromatin. Digestion of nuclei or chromatin with an endogenous rat-liver nuclease (Hewish and Burgoyne 1973) or micrococcal nuclease (for review, see R. D. Kornberg 1977) resulted in the identification and isolation of the nucleosome (the repeating subunit of chromatin), which contains nine histones and on the order of 200 base pairs of DNA.

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The use of nucleases as structural probes has proven to be a very successful approach in studying the organization of chromatin. Digestion of nuclei or chromatin with an endogenous rat-liver nuclease (Hewish and Burgoyne 1973) or micrococcal nuclease (for review, see R. D. Kornberg 1977) resulted in the identification and isolation of the nucleosome (the repeating subunit of chromatin), which contains nine histones and on the order of 200 base pairs of DNA.

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Available abstract

The use of nucleases as structural probes has proven to be a very successful approach in studying the organization of chromatin. Digestion of nuclei or chromatin with an endogenous rat-liver nuclease (Hewish and Burgoyne 1973) or micrococcal nuclease (for review, see R. D. Kornberg 1977) resulted in the identification and isolation of the nucleosome (the repeating subunit of chromatin), which contains nine histones and on the order of 200 base pairs of DNA.

Key concepts: Micrococcal nuclease, Chromatin, Nucleosome, Nuclease, Histone, DNA, Cleavage (geology), Chemistry

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