Artificial diet rearing system for the silkworm Bombyx mori (Lepidoptera: Bombycidae): effect of vitamin C deprivation on larval growth and cocoon production

An artificial diet containing 2% L-ascorbic acid was given to silkworm (Bombyx mori) larvae throughout larval life, or only in some larval instars in order to make a comparison of larvae fed on a diet without L-ascorbic acid throughout larval life. Obtained results show that, when complete L-ascorbic acid deprivation is done during the larval cycle, it affects larval growth and cocoon production. Furthermore, L-ascorbic acid absence from larval food, particularly during the first and last instars, generates beneficial effects to cocoon production without affecting the survival rate or delaying the larval cycle.