A Simple Method to Prepare Methacrylate‐Based Capillary Monolithic Column Using Microwave Irradiation

Abstract A methacrylate‐based monolith was initially prepared in fused‐silica capillary, using microwave irradiation. The electrophoretic and chromatographic behaviors of the prepared capillary monolithic columns were comparatively evaluated in the modes of pressure‐assisted capillary electrochromatography, capillary electrochromatography and low pressure‐assisted liquid chromatography. Baseline separation of the model analytes was, respectively, achieved with the lowest theoretical heights up to 17.0 μm, 28.4 μm and 16.3 μm for thiourea. A scanning electron micrograph of a cross‐section of the capillary column showed that the monolith was formed in the center of the capillary and adhered to the column inner wall.