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Determination of morphine in body fluids by high-performance liquid chromatography with chemiluminescence detection

Richard Abbott, Alan Townshend, R. Gill

Open publisher page 77 citations

Abstract

HPLC has been used in combination with chemiluminescence detection for the determination of morphine in biological fluids. Solid-liquid extraction procedures for the isolation of morphine from whole blood and urine are described, as is the synthesis of the internal standard, N-ethylnormorphine. Improved selectivity and sensitivity over UV detection is apparent. The procedure has a detection limit of 5 ng of morphine on-column after extraction from blood or urine. The utility of the procedure has been tested on forensic case samples.

About this research paper

What this paper is about

HPLC has been used in combination with chemiluminescence detection for the determination of morphine in biological fluids. Solid-liquid extraction procedures for the isolation of morphine from whole blood and urine are described, as is the synthesis of the internal standard, N-ethylnormorphine. Improved selectivity and sensitivity over UV detection is apparent. The procedure has a detection limit of 5 ng of morphine on-column after extraction from blood or urine. The utility of the procedure has been tested on forensic case samples.

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OpenAlex reports 77 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

HPLC has been used in combination with chemiluminescence detection for the determination of morphine in biological fluids. Solid-liquid extraction procedures for the isolation of morphine from whole blood and urine are described, as is the synthesis of the internal standard, N-ethylnormorphine. Improved selectivity and sensitivity over UV detection is apparent. The procedure has a detection limit of 5 ng of morphine on-column after extraction from blood or urine. The utility of the procedure has been tested on forensic case samples.

Key concepts: Chemiluminescence, Chromatography, Detection limit, Chemistry, Urine, Morphine, Extraction (chemistry), High-performance liquid chromatography

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