1992•FEBS LettersRequires access
Expression of functional Thermoplasma acidophilum proteasomes in Escherichia coli
Peter Zwickl, Friedrich Lottspeich, Wolfgang Baumeister
Open publisher page 91 citations
Abstract
The two genes encoding the constituent subunits of the Thermoplasma acidophilum proteasome were expressed in Escherichia coli yielding fully assembled molecules as shown by electron microscopy. The recombinant proteasomes were purified to homogeneity and were shown to have proteolytic activity indistinguishable from proteasomes isolated from T. acidophilum.