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Capillary electrochromatography in columns packed by gravity.Preliminary study

Kimberly J. Reynolds, Luis A. Colón

Open publisher page 30 citations

Abstract

Chromatographic material was delivered into fused silica capillary columns mainly by the use of sedimentation by gravity. After sedimentation of the packing material, the columns were pressurized at 8000 psi and evaluated for capillary electrochromatography (CEC). The capillary columns were tested using a commercially available capillary electrophoresis unit. Under typical CEC conditions, the capillary columns packed by gravity showed reduced plate heights below 2 with a reproducibility of less than 9% RSD.

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What this paper is about

Chromatographic material was delivered into fused silica capillary columns mainly by the use of sedimentation by gravity. After sedimentation of the packing material, the columns were pressurized at 8000 psi and evaluated for capillary electrochromatography (CEC). The capillary columns were tested using a commercially available capillary electrophoresis unit. Under typical CEC conditions, the capillary columns packed by gravity showed reduced plate heights below 2 with a reproducibility of less than 9% RSD.

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Available abstract

Chromatographic material was delivered into fused silica capillary columns mainly by the use of sedimentation by gravity. After sedimentation of the packing material, the columns were pressurized at 8000 psi and evaluated for capillary electrochromatography (CEC). The capillary columns were tested using a commercially available capillary electrophoresis unit. Under typical CEC conditions, the capillary columns packed by gravity showed reduced plate heights below 2 with a reproducibility of less than 9% RSD.

Key concepts: Capillary electrochromatography, Capillary action, Chromatography, Electrochromatography, Capillary electrophoresis, Chemistry, Sedimentation, Reproducibility

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