2006•Journal of Clinical MicrobiologyRequires access

Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of Bordetella pertussis Infection

Kazunari Kamachi, Hiromi Toyoizumi‐Ajisaka, Kohei Toda, Sann Chan Soeung, Svay Sarath, Ya Nareth, Yoshinobu Horiuchi, Kazunobu Kojima, Motohide Takahashi, Yoshichika Arakawa

Open publisher page 85 citations

Abstract

We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.

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Key concepts: Bordetella pertussis, Loop-mediated isothermal amplification, Bordetella, Whooping cough, Microbiology, Virology, Medicine, Biology