1993•Journal of Liquid ChromatographyRequires access
High Performance Liquid Chromatographic Analysis of Lactic Acid and Lactic Acid Lactate in Amrinone Lactate Formulations
Michael J. Lesh, Elizabeth L. Wilkinson, Mark R. Zolfaghari, Mark A. Schreiber
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Abstract
A high performance liquid chromatographic method for the quantitation of lactic acid in Amrinone Lactate formulations is described. Samples were separated using a Dionex HPICE-AS1 ion chromatography exclusion column and a mobile phase of 1 mM sodium octanesulfonate. A conductivity detector equipped with an anion micromembrane suppressor was used and regenerated with 5 mM tetrabutylammonium hydroxide. The method demonstrated a recovery of 100.3% with a linear correlation coefficient of the standards of 0.9999. The selectivity of the method was demonstrated by showing the absence of chromatographic interference by other formulation components. The method also separates lactic acid lactate from lactic acid. Studies conducted on samples of Amrinone Lactate Injection and placebo demonstrated a temperature related equilibrium between lactic acid and lactic acid lactate.