1974•Journal of Applied Polymer ScienceRequires access

Application of an oligomer as an internal standard in gel permeation chromatography

G. N. Patel

Abstract

The usefulness of an oligomer as an internal standard in gel permeation chromatography (GPC) has been investigated under deliberately varied experimental conditions—temperatures of the oven and the siphon, and flow rate. It was demonstrated that the effects of variations in the flow rate and temperature of the siphon on the elution volumes can be significantly minimized, even if not completely eliminated. Use of an oligomer, possibly having a chemical composition similar to that of the samples, is recommended as an internal standard in GPC experiments for better reproducibility and precision.

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Key concepts: Oligomer, Gel permeation chromatography, Elution, Permeation, Chromatography, Reproducibility, Volumetric flow rate, Materials science