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Chemically bonded cyclodextrin stationary phase for the high-performance liquid chromatographic separation and determination of sulphonamides

Abd-El Hamid N. Ahmed, Samia M. El‐Gizawy

Abstract

Mixtures of seven sulphonamides have been separated successfully on a β-cyclodextrin stationary phase by high-performance liquid chromatography (HPLC) using phosphate buffer (pH 7.0)-methanol (85 + 15). The HPLC determination of trisulphapyrimidines and Polysulpha in dosage forms is described. That the proposed method is accurate was established from the percentage recoveries of standard sulphonamide solutions.

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Key concepts: Chromatography, Chemistry, High-performance liquid chromatography, Cyclodextrin, Phosphate buffered saline, Methanol, Stationary phase, Phase (matter)