Anne F. Runge, S. Scott Saavedra, Sergio B. Mendes
Abstract
We describe a rigorous methodology to extract order information of biomolecular assemblies on solid supports using a combination of two polarized evanescent-wave spectroscopies: total internal reflection fluorescence and attenuated total reflection absorbance.
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We describe a rigorous methodology to extract order information of biomolecular assemblies on solid supports using a combination of two polarized evanescent-wave spectroscopies: total internal reflection fluorescence and attenuated total reflection absorbance.
Key concepts: Total internal reflection, Evanescent wave, Absorbance, Optics, Reflection (computer programming), Materials science, Attenuated total reflection, Polarization (electrochemistry)