1979•Preparative BiochemistryRequires access

Preparative Polyacrylamide-Agarose Gel Electrophoresis of Proteins and RNAS by the Use of New Device

Tezro Asao

Abstract

Quantitatively reproducible results were obtained by using a new device for preparation gel electrophoresis combined with polyacrylamide-agarose composite gel. When an adequate gel-buffer system was selected according to the procedure described in this paper, proteins and RNA's were well separated and recovered. The new device for preparative gel electrophoresis and the method for preparation of polyacrylamide-agarose composite gel are presented together with the elution profiles of the recovered substances.

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Key concepts: Agarose, Gel electrophoresis of nucleic acids, Polyacrylamide gel electrophoresis, Chromatography, Color marker, Gel electrophoresis of proteins, Polyacrylamide, Two-dimensional gel electrophoresis