1995Journal of PhytopathologyRequires access

Structures of Lipopolysaccharide Side‐chains of NCPPB 871, the Neopathotype Strain of Pseudomonas syringae pv. aptata, Strain 299A of pv. pisi and NCPPB 52, the Neopathotype Strain of pv. phaseolicola, in Relation to O‐serogroup

Mariangela Spitali, N.H. Rees, Robin Wait, A. R. W. Smith

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Abstract

Abstract The side‐chains of lipopolysaccharides from Pseudomonas syringae pvs. aptata, pisi and phaseolicola contain D‐rhamnose. Using 1H‐and 13C‐nuclear magnetic resonance spectroscopy and methylation analysis, the side‐chains of NCPPB 871, the neopathotype strain of pv. aptata, and strain 299A of pv. pisi race 1 were found to be of identical structure: image Side‐chains from NCPPB 52, the neopathotype strain of pv. phaseolicola, were based on the same backbone structure, but were substituted to the extent of approximately 67% with branch D‐fucose residues: image Side‐chain structures are correlated with a previously proposed serouping system.

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Abstract The side‐chains of lipopolysaccharides from Pseudomonas syringae pvs. aptata, pisi and phaseolicola contain D‐rhamnose. Using 1H‐and 13C‐nuclear magnetic resonance spectroscopy and methylation analysis, the side‐chains of NCPPB 871, the neopathotype strain of pv. aptata, and strain 299A of pv. pisi race 1 were found to be of identical structure: image Side‐chains from NCPPB 52, the neopathotype strain of pv. phaseolicola, were based on the same backbone structure, but were substituted to the extent of approximately 67% with branch D‐fucose residues: image Side‐chain structures are correlated with a previously proposed serouping system.

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Available abstract

Abstract The side‐chains of lipopolysaccharides from Pseudomonas syringae pvs. aptata, pisi and phaseolicola contain D‐rhamnose. Using 1H‐and 13C‐nuclear magnetic resonance spectroscopy and methylation analysis, the side‐chains of NCPPB 871, the neopathotype strain of pv. aptata, and strain 299A of pv. pisi race 1 were found to be of identical structure: image Side‐chains from NCPPB 52, the neopathotype strain of pv. phaseolicola, were based on the same backbone structure, but were substituted to the extent of approximately 67% with branch D‐fucose residues: image Side‐chain structures are correlated with a previously proposed serouping system.

Key concepts: Pseudomonas syringae, Strain (injury), Pseudomonadales, Side chain, Pseudomonadaceae, Rhamnose, Chemistry, Microbiology

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Structures of Lipopolysaccharide Side‐chains of NCPPB 871, the Neopathotype Strain of Pseudomonas syringae pv. aptata, Strain 299A of pv. pisi and NCPPB 52, the Neopathotype Strain of pv. phaseolicola, in Relation to O‐serogroup — Research Paper | ScholarLens