Chiral Resolution of Basic Pharmaceutical Enantiomers by Capillary Zone Electrophoresis

Enantioseparations of four basic pharmaceutical enantiomers i.e, (±)-threo-1-p-nitrophenyl-2-amino-1,3-propanediol (NAPD), (±)-threo-1-p-methysulfonyl serine ethylester (MSSE), fluoxetine and carteolol were performed by capillary zone electrophoresis using carboxylmethyl-β-cyclodextrin (CM-β-CD), hydroxypropyl-β-cyclodextrin (HP-β-CD) and dimethyl-β-cyclodextrin (DM-β-CD) as chiral selectors. The type and concentration of derivatized CDs, buffer composition and buffer pH, organic modifier, running voltage and capillary temperature were selected as variable parameters, their effects on chiral resolution were studied and optimized. CM-β-CD turns out to give optimal resolution for four enantiomers. The simple, fast and sensitive methods for highly efficient chiral separation for these four enantiomers were established. Analytical calibration equation, detection limit and recovery were determined.