1991FEBS LettersOpen access

Chlorophyll levels in the pigment‐binding proteins of photosystem II A study based on the chlorophyll to cytochrome ratio in different photosystem II preparations

Roberto Barbato, Helen L. Race, Giulia Friso, James Barber

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Abstract

The chlorophyll levels in pigment proteins of photosystem II were investigated by using photosystem II preparations with different levels of complexity. Based on the assumption that there is 1 cytochrome b559 per reaction centre it has been found that oxygen-evolving complexes containing CP26 and CP29 bind 42 chlorophyll molecules. When CP26 and CP29 are stripped away, the resulting PSII cores bind 30 chlorophyll molecules while CP43-less cores bind approximately 18 chlorophylls. It is therefore concluded that CP47 and CP43 bind 9-12 molecules of chlorophyll a and the D1/D2 complex binds 6 chlorophylls. Taken together CP26 and CP29 bind about 12 chlorophyll molecules.

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The chlorophyll levels in pigment proteins of photosystem II were investigated by using photosystem II preparations with different levels of complexity. Based on the assumption that there is 1 cytochrome b559 per reaction centre it has been found that oxygen-evolving complexes containing CP26 and CP29 bind 42 chlorophyll molecules. When CP26 and CP29 are stripped away, the resulting PSII cores bind 30 chlorophyll molecules while CP43-less cores bind approximately 18 chlorophylls. It is therefore concluded that CP47 and CP43 bind 9-12 molecules of chlorophyll a and the D1/D2 complex binds 6 chlorophylls. Taken together CP26 and CP29 bind about 12 chlorophyll molecules.

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Available abstract

The chlorophyll levels in pigment proteins of photosystem II were investigated by using photosystem II preparations with different levels of complexity. Based on the assumption that there is 1 cytochrome b559 per reaction centre it has been found that oxygen-evolving complexes containing CP26 and CP29 bind 42 chlorophyll molecules. When CP26 and CP29 are stripped away, the resulting PSII cores bind 30 chlorophyll molecules while CP43-less cores bind approximately 18 chlorophylls. It is therefore concluded that CP47 and CP43 bind 9-12 molecules of chlorophyll a and the D1/D2 complex binds 6 chlorophylls. Taken together CP26 and CP29 bind about 12 chlorophyll molecules.

Key concepts: Photosystem II, Chlorophyll, Light-harvesting complexes of green plants, Photosystem I, Chlorophyll a, Chemistry, P700, Pigment

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