ScholarLens Back to search
1981•Drug Development and Industrial PharmacyRequires access

Direct Radioimmunoassay for Determination of Betamethasone 17-Benzoate

Michael C. Theodorakis, S. V. S. Stefanakou

Open publisher page 2 citations

Abstract

A direct radioimmunoassay for the determination of betamethasone 17-benzoate (I) in serum was developed. In this assay, a highly specific antibody is used to bind the 125I-labeled antigen. Polyethylene glycol is used for the separation of the free from bound antigen. A blocking agent (ANS) was used to inhibit binding of the steroid onto serum proteins. The minimum detectable concentration was 15 pg/ml. The intra and inter assay coefficients of variation were 7.4% and 10.2%, respectively. This assay required 0.1 ml of serum and it did not require extraction or chromatographic separation of the steroid.

About this research paper

What this paper is about

A direct radioimmunoassay for the determination of betamethasone 17-benzoate (I) in serum was developed. In this assay, a highly specific antibody is used to bind the 125I-labeled antigen. Polyethylene glycol is used for the separation of the free from bound antigen. A blocking agent (ANS) was used to inhibit binding of the steroid onto serum proteins. The minimum detectable concentration was 15 pg/ml. The intra and inter assay coefficients of variation were 7.4% and 10.2%, respectively. This assay required 0.1 ml of serum and it did not require extraction or chromatographic separation of the steroid.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A direct radioimmunoassay for the determination of betamethasone 17-benzoate (I) in serum was developed. In this assay, a highly specific antibody is used to bind the 125I-labeled antigen. Polyethylene glycol is used for the separation of the free from bound antigen. A blocking agent (ANS) was used to inhibit binding of the steroid onto serum proteins. The minimum detectable concentration was 15 pg/ml. The intra and inter assay coefficients of variation were 7.4% and 10.2%, respectively. This assay required 0.1 ml of serum and it did not require extraction or chromatographic separation of the steroid.

Key concepts: Radioimmunoassay, Betamethasone, Chromatography, Chemistry, Polyethylene glycol, Steroid, Antigen, Biochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Direct Radioimmunoassay for Determination of Betamethasone 17-Benzoate — Research Paper | ScholarLens