Preliminary Analysis of Bacterial Flora in Turbot Scophthalmus maximus Cultured in Deep Well Seawater

The microbiological characters and composition of bacterial flora in fresh turbot Scophthalmus maximus were observed with qualitative and quantitative analysis. Samples of turbot fish were collected in the spring, summer and autumn of one year. The total amounts of bacteria from the culturing water, gills and skin were quantified as 3.46±2.4-3.82±1.2 log10cfu·ml-1, 5.19±2.3-6.28±1.7 log10cfu·ml-1 and 2.70±1.1-2.95±2.8 log10cfu·ml-1, respectively. The isolated strains and bacterial flora were identified and classified. The results showed that among 206 isolated strains, Gram-positive bacteria accounted for 18.9% of the total flora, and Gram-negative bacteria formed 82.4%, including predominant bacteria such as Staphylococcusaureus (12.6%), Serratia liquefaciens (10.7%), Enterobacter (19.4%) and Pseudomonas (8.3%). A large number of isolated Enterobacter indicated that the culturing water was polluted. We report here for the first time the microbial flora in the turbot cultured in deep well seawater of Liaodong Penisula, which provides a preliminary basis for the subsequent study on the specific spoilage organisms from chilled turbot fish.