1985•MicrobiologyRequires access

Molecular Cloning of a Bacillus subtilis Gene Involved in Spore Outgrowth

E Ferrari, F. Scoffone, Giovanni Ciarrocchi, A. Galizzi

Abstract

A lambda Charon 4A derivative carrying the outB gene of Bacillus subtilis has been identified by transformation of a B. subtilis mutant temperature-sensitive in spore outgrowth. The cloned region is a single EcoRI fragment 14 kb in length. In addition to outB, the cloned DNA includes at least part of the amyE and aroI loci.

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Key concepts: Bacillus subtilis, EcoRI, Molecular cloning, Transformation (genetics), Mutant, Gene, Biology, Genetics