1971•Transactions of the Royal Society of Tropical Medicine and HygieneRequires access
Experimental infection of Aedes aegypti and Aedes albopictus with dengue viruses
Robert H. Whitehead, Thomas M. Yuill, Douglas J. Gould, Phinit Simasathien
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Abstract
The susceptibility of two Aedes (Stegomyia) species, Ae. aegypti and Ae. albopictus, to infection with dengue virus was assessed using both natural feeding on a viraemic gibbon and a membrane feeding technique. Both species were highly susceptible to dengue virus infection. It was found that both species were more sensitive than the LLC-MK2 cell plaque assay system used in detecting viraemia in a gibbon infected with a low tissue culture passage strain of dengue-2 virus. However, the same strain passaged in LLC-MK2 cells failed to infect either species when the mosquitoes were fed upon a suspension of this virus. Both species were infected readily when fed upon a suspension of a mouse adapted strain of dengue-2 virus.