AN ESCHERICHIA COLI MUTANT WITH TRYPTOPHANASE SYNTHESIS SPECIFICALLY INSENSITIVE TO CATABOLITE REPRESSION AND THE SIGNIFICANCE OF INTRACELLULAR PHOSPHOENOLPYRUVATE POOLS

Two strains of catabolite repression-insensitive mutant, TAB40-303 and TAB40-203, were isolated. TAB40-303 was a mutant not specific for tryptophanase synthesis and seemed to be injured at a point in glucose catabolism. Tryptophanase formation by TAB40-203 was not influenced by any carbon sources and not affected by adenosine 3′, 5′-cyclic monophosphate (c-AMP) which were effective in the parent strain. The mutant was presumed to be injured at the site at which c-AMP acted. Phosphoenolpyruvate (PEP) was again shown to be closely related to the catabolite repression by using these mutants.