2006•Annals of the New York Academy of SciencesOpen access

Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell‐Entry Assay

Jinying Ge, Zhiyun Wen, Xijun Wang, Sen Hu, Yonggang Liu, Xiangang Kong, Hualan Chen, Zhigao Bu

Abstract

We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Delta G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS-CoV in trans. The neutralization and infection blocking tests showed that the VSV Delta G*SG and SARS-CoV reacted similarly to SARS-CoV specific antiserum, suggesting the VSVDelta G*SG can be a safe replacement of the live SARS-CoV for neutralization test and cell-entry assay.

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Key concepts: Vesicular stomatitis virus, Virology, Coronavirus, Vesicular stomatitis Indiana virus, Glycoprotein, Neutralization, Rhabdoviridae, Respiratory system