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2005•Journal of Chinese Integrative MedicineRequires access

Determination of baicalin in Xiaoyanling Decoction by high-performance liquid chromatography

Junyi Jiang

Open publisher page 2 citations

Abstract

OBJECTIVE: To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction. METHODS: HPLC method was used for determination. A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water (H(2)O:H(3)PO(4)=53:0.2) 40:60, and the detective wavelength was 277 nm. RESULTS: The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml (RSD<5%). CONCLUSION: The content of baicalin in Xiaoyanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.

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What this paper is about

OBJECTIVE: To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction. METHODS: HPLC method was used for determination. A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water (H(2)O:H(3)PO(4)=53:0.2) 40:60, and the detective wavelength was 277 nm. RESULTS: The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml (RSD<5%). CONCLUSION: The content of baicalin in Xiaoyanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.

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Available abstract

OBJECTIVE: To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction. METHODS: HPLC method was used for determination. A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water (H(2)O:H(3)PO(4)=53:0.2) 40:60, and the detective wavelength was 277 nm. RESULTS: The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml (RSD<5%). CONCLUSION: The content of baicalin in Xiaoyanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.

Key concepts: Baicalin, Decoction, High-performance liquid chromatography, Chromatography, Chemistry, Traditional medicine, Medicine

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